Interprofessionality in the health professions in the transformation of a modern role and profession development. Report on the results of a world café at the Drei-Länder-Tagung on May 5, 2022 in Bern / Interprofessionalität in den Gesundheitsberufen im Wandel einer modernen Rollen- und Professionsentwicklung. Bericht über die Ergebnisse eines World Cafés auf der Drei-Länder-Tagung am 5. Mai 2022 in Bern

The health professions in the German-speaking region of Germany, Austria and Switzerland, the so-called D-A-CH area, are in dynamic phases of their professionalization due to their shift towards education at universities. In addition, the needs of current and future health care require all health professionals (HPs) to have collaborative competencies and a willingness to work together. This willingness and the challenge of co-creation of health systems by all health professionals is a good starting point for the evolution of the professions. So what must professional profiles, role beliefs - in short, modern professionalization strategies - be like so that, at the beginning of the 21st century, education and professional practice do not remain stuck in old thought structures and templates and in monodisciplinary „silo thinking.” What does it mean in concrete terms for the professionalization of professions that health care professions must (be able to) increasingly work together? Doesn’t professionalization so far tend to mean exclusivity and isn’t a stronger demarcation between the professions then the consequence? Against the background of professionalization through academization, do university studies not tend to aim at a stronger demarcation from other disciplines and professions? Professionalization includes exclusive competencies and specialized methods against the background of one's own profession-related action sciences. At the three-country meeting of the VFWG in Bern on 5–6 May 2022, this area of tension was put up for discussion in a World Café based on four theses. The following article documents the results of the individual discussion rounds and aims to stimulate a critical discours

Inhibition of the Soluble Epoxide Hydrolase Promotes Albuminuria in Mice with Progressive Renal Disease

Epoxyeicotrienoic acids (EETs) are cytochrome P450-dependent anti-hypertensive and anti-inflammatory derivatives of arachidonic acid, which are highly abundant in the kidney and considered reno-protective. EETs are degraded by the enzyme soluble epoxide hydrolase (sEH) and sEH inhibitors are considered treatment for chronic renal failure (CRF). We determined whether sEH inhibition attenuates the progression of CRF in the 5/6-nephrectomy model (5/6-Nx) in mice. 5/6-Nx mice were treated with a placebo, an ACE-inhibitor (Ramipril, 40 mg/kg), the sEH-inhibitor cAUCB or the CYP-inhibitor fenbendazole for 8 weeks. 5/6-Nx induced hypertension, albuminuria, glomerulosclerosis and tubulo-interstitial damage and these effects were attenuated by Ramipril. In contrast, cAUCB failed to lower the blood pressure and albuminuria was more severe as compared to placebo. Plasma EET-levels were doubled in 5/6 Nx-mice as compared to sham mice receiving placebo. Renal sEH expression was attenuated in 5/6-Nx mice but cAUCB in these animals still further increased the EET-level. cAUCB also increased 5-HETE and 15-HETE, which derive from peroxidation or lipoxygenases. Similar to cAUCB, CYP450 inhibition increased HETEs and promoted albuminuria. Thus, sEH-inhibition failed to elicit protective effects in the 5/6-Nx model and showed a tendency to aggravate the disease. These effects might be consequence of a shift of arachidonic acid metabolism into the lipoxygenase pathway

Interprofessionality in the health professions in the transformation of a modern role and profession development. Report on the results of a world café at the Drei-Länder-Tagung on May 5, 2022 in Bern / Interprofessionalität in den Gesundheitsberufen im Wandel einer modernen Rollen- und Professionsentwicklung. Bericht über die Ergebnisse eines World Cafés auf der Drei-Länder-Tagung am 5. Mai 2022 in Bern

The health professions in the German-speaking region of Germany, Austria and Switzerland, the so-called D-A-CH area, are in dynamic phases of their professionalization due to their shift towards education at universities. In addition, the needs of current and future health care require all health professionals (HPs) to have collaborative competencies and a willingness to work together. This willingness and the challenge of co-creation of health systems by all health professionals is a good starting point for the evolution of the professions. So what must professional profiles, role beliefs - in short, modern professionalization strategies - be like so that, at the beginning of the 21st century, education and professional practice do not remain stuck in old thought structures and templates and in monodisciplinary „silo thinking.” What does it mean in concrete terms for the professionalization of professions that health care professions must (be able to) increasingly work together? Doesn’t professionalization so far tend to mean exclusivity and isn’t a stronger demarcation between the professions then the consequence? Against the background of professionalization through academization, do university studies not tend to aim at a stronger demarcation from other disciplines and professions? Professionalization includes exclusive competencies and specialized methods against the background of one's own profession-related action sciences. At the three-country meeting of the VFWG in Bern on 5–6 May 2022, this area of tension was put up for discussion in a World Café based on four theses. The following article documents the results of the individual discussion rounds and aims to stimulate a critical discours

Interprofessionalität in den Gesundheitsberufen im Wandel einer modernen Rollen- und Professionsentwicklung. Bericht über die Ergebnisse eines World Cafés auf der Drei-Länder-Tagung am 5. Mai 2022 in Bern

Die Gesundheitsberufe im deutschsprachigen Raum von Deutschland, Österreich und Schweiz, dem sog. D-A-CH-Raum befinden sich aufgrund ihres Wechsels hin zu Ausbildungen an Hochschulen in dynamischen Phasen ihrer Professionalisierung. Zudem erfordern die Bedarfe aktueller und künftiger Gesundheitsversorgung von allen Health Professionals (HPs) kollaborative Kompetenzen und die Bereitschaft zusammenzuarbeiten. Dieser Wille und die Herausforderung der Mitgestaltung der Gesundheitssysteme durch alle Gesundheitsberufe ist ein guter Ausgangspunkt für die Weiterentwicklung der Berufe. Wie müssen also Berufsprofile, Rollenüberzeugungen – kurz: moderne Professionalisierungsstrategien sein, damit am Beginn des 21. Jahrhunderts Bildung und Berufsausübung nicht in alten Denkstrukturen und -schablonen sowie in einem monodisziplinären “Silodenken” verhaften bleiben. Was bedeutet es konkret für die Professionalisierung von Berufen, dass Gesundheitsberufe vermehrt zusammenarbeiten (können) müssen? Bedeutet Professionalisierung bisher nicht tendenziell Exklusivität und ist dann eine stärkere Grenzziehung zwischen den Berufen nicht die Folge? Zielt Hochschulstudium vor dem Hintergrund einer rofessionalisierung durch Akademisierung nicht tendenziell auf eine stärkere Abgrenzung von anderen Fachdisziplinen und -professionen ab? Professionalisierung inkludiert exklusive Kompetenzen und spezialisierte Methoden vor dem Hintergrund der eigenen berufsbezogenen Handlungswissenschaften. Auf der Drei-Länder-Tagung des Vereins zur Förderung der Wissenschaft in den Gesundheitsberufen VFWG am 5./6. Mai 2022 wurde dieses Spannungsfeld im Rahmen eines World Cafés anhand von vier Thesen zur Diskussion gestellt. Der folgende Beitrag dokumentiert die Ergebnisse der einzelnen Diskussionsrunden mit dem Ziel, einen kritischen Diskurs anzuregen

Pleiotropic effects of laminar flow and statins depend on the Krüppel-like factor-induced lncRNA MANTIS

AIMS: To assess the functional relevance and therapeutic potential of the pro-angiogenic long non-coding RNA MANTIS in vascular disease development. METHODS AND RESULTS: RNA sequencing, CRISPR activation, overexpression, and RNAi demonstrated that MANTIS, especially its Alu-element, limits endothelial ICAM-1 expression in different types of endothelial cells. Loss of MANTIS increased endothelial monocyte adhesion in an ICAM-1-dependent manner. MANTIS reduced the binding of the SWI/SNF chromatin remodelling factor BRG1 at the ICAM-1 promoter. The expression of MANTIS was induced by laminar flow and HMG-CoA-reductase inhibitors (statins) through mechanisms involving epigenetic rearrangements and the transcription factors KLF2 and KLF4. Mutation of the KLF binding motifs in the MANTIS promoter blocked the flow-induced MANTIS expression. Importantly, the expression of MANTIS in human carotid artery endarterectomy material was lower compared with healthy vessels and this effect was prevented by statin therapy. Interestingly, the protective effects of statins were mediated in part through MANTIS, which was required to facilitate the atorvastatin-induced changes in endothelial gene expression. Moreover, the beneficial endothelial effects of statins in culture models (spheroid outgrowth, proliferation, telomerase activity, and vascular organ culture) were lost upon knockdown of MANTIS. CONCLUSION: MANTIS is tightly regulated by the transcription factors KLF2 and KLF4 and limits the ICAM-1 mediated monocyte adhesion to endothelial cells and thus potentially atherosclerosis development in humans. The beneficial effects of statin treatment and laminar flow are dependent on MANTIS

Pleiotropic effects of laminar flow and statins depend on the Kruppel-like factor-induced lncRNA MANTIS

Aims To assess the functional relevance and therapeutic potential of the pro-angiogenic long non-coding RNA MANTIS in vascular disease development. Methods and results RNA sequencing, CRISPR activation, overexpression, and RNAi demonstrated that MANTIS, especially its Aluelement, limits endothelial ICAM-1 expression in different types of endothelial cells. Loss of MANTIS increased endothelial monocyte adhesion in an ICAM-1-dependent manner. MANTIS reduced the binding of the SWI/SNF chromatin remodelling factor BRG1 at the ICAM-1 promoter. The expression of MANTIS was induced by laminar flow and HMG-CoA-reductase inhibitors (statins) through mechanisms involving epigenetic rearrangements and the transcription factors KLF2 and KLF4. Mutation of the KLF binding motifs in the MANTIS promoter blocked the flow-induced MANTIS expression. Importantly, the expression of MANTIS in human carotid artery endarterectomy material was lower compared with healthy vessels and this effect was prevented by statin therapy. Interestingly, the protective effects of statins were mediated in part through MANTIS, which was required to facilitate the atorvastatin-induced changes in endothelial gene expression. Moreover, the beneficial endothelial effects of statins in culture models (spheroid outgrowth, proliferation, telomerase activity, and vascular organ culture) were lost upon knockdown of MANTIS. Conclusion MANTIS is tightly regulated by the transcription factors KLF2 and KLF4 and limits the ICAM-1 mediated monocyte adhesion to endothelial cells and thus potentially atherosclerosis development in humans. The beneficial effects of statin treatment and laminar flow are dependent on MANTIS

Blood pressure and albuminuria.

<p>Blood pressure measured by tail cuff technique (A) and albuminuria (B) were determined 4 and 8 weeks after initiation of treatment. Effects of placebo, sEH-inhibition by cAUCB, ACE-inhibition by ramipril (Ramip.) and CYP-inhibition by fenbendazole (Fenbe.) are shown on sham operated (−) and 5-6-Nx (+) animals. * p<0,05 vs. sham, # p<0,05 vs. placebo (n = 10–12/group).</p

Plasma levels of hydroxyeicosatetraenoic (HETE) acids.

<p>Plasma level of 5-HETE, 12-HETE, 15-HETE and 20-HETE as determined by LC-MS/MS 8 weeks after initiation of treatment. Effects of placebo, sEH-inhibition by cAUCB, ACE-inhibition by ramipril (Ramip.) and CYP-inhibition by fenbendazole (Fenbe.) are shown on sham operated (−) and 5-6-Nx (+) animals. * p<0,05 vs. sham, # p<0,05 vs. placebo, § p<0.05 vs. placebo all groups (n = 5–8/group).</p

Histological tubulointerstitial changes.

<p>Representative kidney sections (magnification ×100) stained by Sirius Red in phase contrast light microscopy and polarized light microscopy scoring tubulointerstitial damage index (TSI) and interstitial fibrosis after 8 weeks of treatment. Effects of placebo, sEH-inhibition by cAUCB, ACE-inhibition by ramipril (Ramip.) and CYP-inhibition by fenbendazole (Fenbe.) are shown on sham operated (−) and 5-6-Nx (+) animals. * p<0,05 vs. sham, # p<0,05 vs. placebo (n = 5–8/group). The scale bar denotes 100 µmeter.</p